This mode is used to converts a compact alignment to a read set file. It is implemented by edu.cornell.med.icb.goby.modes.CompactAlignmentToReadSetMode.java.
Mode Parameters
The following options are available in this mode
| Flag | Arguments | Required | Description |
|---|---|---|---|
n/a | input | yes | The compact alignment file(s) to read as input. |
(-s|--suffix) | suffix | no | The suffix to use to construct the read set filename with the input basename. Together with input basename, will determine where the output is written. |
--matching-reads | n/a | no | When provided, output reads that matched the reference. |
--non-matching-reads | n/a | no | When provided, output reads that did not match the reference. |
--ambiguous-reads | n/a | no | When provided, output reads that matched more than k locations in the reference. |
--non-ambiguous-reads | n/a | no | When provided, output reads that matched more than k locations in the reference. |
--ambiguity-threshold | ambiguity-threshold | no | The parameter k, threshold used to determine if a read should be considered ambiguous. The value of k is the number of locations that a read matches in the reference. |
(-f|--pre-filter) | pre-filter | no | A read set to use as pre-filter. Reads must be in the pre-filter to be in the written to the output read set. |

Leave a Comment